The CCND1 Polyclonal Antibody (CAB11310) is a vital tool for researchers studying the CCND1 gene, which encodes cyclin D1, a protein known for its involvement in cell cycle progression and cancer development. This antibody, produced in rabbits, exhibits high specificity and sensitivity for human samples, making it suitable for use in various research applications such as Western blot and immunohistochemistry.Cyclin D1 is a key regulator of cell division and proliferation, with dysregulation of its expression often associated with tumor formation and progression. By targeting the CCND1 protein, researchers can gain insights into the mechanisms underlying cancer development and potentially identify novel therapeutic strategies for treating various cancers.
Furthermore, the CCND1 Polyclonal Antibody is essential for investigating the role of cyclin D1 in other cellular processes beyond cell cycle regulation, such as DNA replication, transcriptional activation, and apoptosis. Its versatility and reliability make it an invaluable tool for studies in oncology, cell biology, and molecular biology, providing crucial insights into the mechanisms driving cancer progression and potential targets for anti-cancer therapies.
Product Name:
Cyclin D1 Rabbit Polyclonal Antibody
SKU:
CAB11310
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-295 of human Cyclin D1 (NP_444284.1).
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers.
Purification Method:
Affinity purification
Gene ID:
595
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Cyclin D1 Rabbit pAb (CAB11310) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 2s.
