The CCND1 Polyclonal Antibody (CAB11022) is a valuable tool for researchers studying the CCND1 protein, which plays a crucial role in cell cycle regulation and cell proliferation. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.CCND1, also known as cyclin D1, is a key regulator of the G1/S phase transition in the cell cycle, promoting cell division and growth. Dysregulation of CCND1 has been implicated in various cancers, making it an important target for cancer research. By binding specifically to the CCND1 protein, this antibody enables the detection and analysis of CCND1 expression in different cell types, making it ideal for studies in oncology and cell biology.
Understanding the function and regulation of CCND1 is essential for developing targeted therapies and interventions for cancer and other diseases characterized by abnormal cell proliferation. The CCND1 Polyclonal Antibody provides a reliable tool for researchers to investigate the role of CCND1 in physiological and pathological processes, advancing our knowledge of cell cycle control and potential therapeutic strategies.
Product Name:
Cyclin D1 Rabbit Polyclonal Antibody
SKU:
CAB11022
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 200-295 of human Cyclin D1 (NP_444284.1).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
BCL1; PRAD1; U21B31; D11S287E; Cyclin D1
Positive Sample:
MCF7,NIH/3T3,C6
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Membrane, Nucleus.
Calculated MW:
34kDa
Observed MW:
35kDa
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers.
Purification Method:
Affinity purification
Gene ID:
595
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Cyclin D1 Rabbit pAb (CAB11022) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
