Anti-Cyclin D1 Antibody (CAB0310)
- SKU:
- CAB0310
- Product Type:
- Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
Anti-Cyclin D1 Antibody (CAB0310)
The CCND1 Polyclonal Antibody (CAB0310) is a valuable tool for researchers studying the CCND1 protein, a key regulator of cell cycle progression. This antibody, produced in rabbits, shows high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to CCND1, this antibody enables accurate detection and analysis of the protein in a variety of cell types, making it an essential tool for studies in cell biology, cancer research, and drug development.CCND1, also known as cyclin D1, plays a crucial role in regulating the G1 to S phase transition of the cell cycle.
Dysregulation of CCND1 has been implicated in various cancers, making it a promising therapeutic target for cancer treatment. Understanding the function and expression of CCND1 is essential for uncovering its role in tumorigenesis and developing targeted therapies to combat cancer progression. The CCND1 Polyclonal Antibody provides researchers with a reliable means to investigate the biology of CCND1 and its potential as a therapeutic target.
| Antibody Name: | Anti-Cyclin D1 Antibody |
| Antibody SKU: | CAB0310 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 96-295 of human Cyclin D1 (NP_444284.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:1000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Rat |
| Positive Samples: | Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 96-295 of human Cyclin D1 (NP_444284.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KSRL QLLG ATCM FVAS KMKE TIPL TAEK LCIY TDNS IRPE ELLQ MELL LVNK LKWN LAAM TPHD FIEH FLSK MPEA EENK QIIR KHAQ TFVA LCAT DVKF ISNP PSMV AAGS VVAA VQGL NLRS PNNF LSYY RLTR FLSR VIKC DPDC LRAC QEQI EALL ESSL RQAQ QNMD PKAA EEEE EEEE EVDL ACTP TDVR DVDI |
| Gene ID: | 595 |
| Uniprot: | P24385 |
| Cellular Location: | Cytoplasm, Membrane, Nucleus |
| Calculated MW: | 33kDa |
| Observed MW: | 34kDa |
| Synonyms: | BCL1, D11S287E, PRAD1, U21B31, CCND1, Cyclin D1, cyclin D1 |
| Background: | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. |
| UniProt Protein Function: | CCND1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. |
| UniProt Protein Details: | Protein type:Activator; Cell cycle regulation; Nuclear receptor co-regulator; Oncoprotein Chromosomal Location of Human Ortholog: 11q13.3 Cellular Component: cyclin-dependent protein kinase holoenzyme complex; cytosol; intracellular; nucleoplasm; nucleus; transcriptional repressor complex Molecular Function:enzyme binding; histone deacetylase binding; protein binding; protein kinase binding; transcription corepressor activity; transcription factor binding Biological Process: G1 DNA damage checkpoint; G1/S transition of mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell cycle; positive regulation of cyclin-dependent protein kinase activity; positive regulation of protein amino acid phosphorylation; response to DNA damage stimulus; response to drug Disease: Myeloma, Multiple; Von Hippel-lindau Syndrome |
| NCBI Summary: | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P24385 |
| NCBI GenInfo Identifier: | 116152 |
| NCBI Gene ID: | 595 |
| NCBI Accession: | P24385.1 |
| UniProt Secondary Accession: | P24385,Q6LEF0, |
| UniProt Related Accession: | P24385 |
| Molecular Weight: | 34kDa |
| NCBI Full Name: | G1/S-specific cyclin-D1 |
| NCBI Synonym Full Names: | cyclin D1 |
| NCBI Official Symbol: | CCND1Â Â |
| NCBI Official Synonym Symbols: | BCL1; PRAD1; U21B31; D11S287EÂ Â |
| NCBI Protein Information: | G1/S-specific cyclin-D1 |
| UniProt Protein Name: | G1/S-specific cyclin-D1 |
| UniProt Synonym Protein Names: | B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; PRAD1 oncogene |
| Protein Family: | G1/S-specific cyclin |
| UniProt Gene Name: | CCND1Â Â |
 | Western blot analysis of extracts of rat kidney, using Cyclin D1 antibody (CAB0310) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using Cyclin D1 antibody (CAB0310) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human esophagus using Cyclin D1 antibody (CAB0310) at dilution of 1:100 (40x lens). |
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