The CCNB1 Polyclonal Antibody (CAB2056) is a crucial tool for researchers studying the CCNB1 protein, a key regulator of cell cycle progression and division. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to CCNB1, this antibody enables precise detection and analysis of the protein in various cell types, offering valuable insights for studies in cancer biology and cell cycle regulation.CCNB1, also known as cyclin B1, is essential for the activation of cyclin-dependent kinase 1 (CDK1) in the G2/M phase transition of the cell cycle.
Dysregulation of CCNB1 expression or activity is associated with uncontrolled cell proliferation and tumorigenesis, making it a significant target for cancer research and therapeutic development. By understanding the role of CCNB1 in cell cycle regulation, researchers can uncover potential therapeutic strategies for treating cancer and other proliferative disorders.
Product Name:
Cyclin B1 Rabbit Polyclonal Antibody
SKU:
CAB2056
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 334-433 of human Cyclin B1 (NP_114172.1).
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle.
Purification Method:
Affinity purification
Gene ID:
891
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Cyclin B1 antibody (CAB2056) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
