The CCNB1 Polyclonal Antibody (CAB16038) is a valuable tool for researchers studying the cell cycle regulator Cyclin B1 (CCNB1). Cyclin B1 is a key player in the control of cell division and has been implicated in various cellular processes like DNA replication and mitosis. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.By targeting the CCNB1 protein, researchers can effectively detect and analyze its expression in a variety of cell types, making it ideal for investigations in cell biology and cancer research.
Understanding the role of CCNB1 in regulating cell cycle progression is essential for identifying potential therapeutic targets for diseases characterized by uncontrolled cell growth, such as cancer.Overall, the CCNB1 Polyclonal Antibody is a reliable tool for studying the function of Cyclin B1 and its implications in cellular processes and disease development. Its specificity and versatility make it a valuable asset for researchers exploring the intricate mechanisms of cell cycle regulation.
Product Name:
Cyclin B1 Rabbit Polyclonal Antibody
SKU:
CAB16038
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 334-433 of human Cyclin B1 (NP_114172.1).
WB,1:100 - 1:500 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
CCNB; Cyclin B1
Positive Sample:
C2C12,C6
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:
48kDa
Observed MW:
55kDa
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle.
Purification Method:
Affinity purification
Gene ID:
891
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using Cyclin B1 Rabbit pAb (CAB16038) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
