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Cyclin A1 Rabbit Polyclonal Antibody (CAB14529)

SKU:
CAB14529
Product Type:
Antibody
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Cyclin A1 Rabbit Polyclonal Antibody
SKU:CAB14529
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 350-450 of human CCNA1 (NP_001104515.1).
Sequence:YLRR QGVC VRTE NLAK YVAE LSLL EADP FLKY LPSL IAAA AFCL ANYT VNKH FWPE TLAA FTGY SLSE IVPC LSEL HKAY LDIP HRPQ QAIR EKYK ASKY L
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:CT146; Cyclin A1
Positive Sample:Mouse brain,Mouse testis,Mouse heart,Rat brain,Rat testis,Rat heart
Conjugate:Unconjugated
Cellular Localization:Nucleus.
Calculated MW:52kDa
Observed MW:52kDa

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:8900
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Cyclin A1 Rabbit pAb (CAB14529) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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