The Cbln1 Polyclonal Antibody (PACO22256) is a valuable tool for researchers investigating Cbln1, a protein involved in synaptic function and neurobiology. This antibody, produced in rabbits, is specifically reactive with human samples and has been validated for use in various applications, including Western blotting.Cbln1 plays a crucial role in mediating synaptic connections and plasticity in the brain, making it a key player in neurological research. Its involvement in regulating neural circuits and synaptic transmission underscores its importance in studies related to neurodevelopmental disorders, neurodegenerative diseases, and cognitive function.
By targeting the Cbln1 protein, this antibody allows for the detection and analysis of Cbln1 expression in different cell types and tissues, offering insights into its role in neuronal communication and brain function. Researchers in neuroscience and related fields will find this antibody essential for investigating the intricate mechanisms underlying brain development and function.
Antibody Name:
CBLN1 Antibody (PACO22256)
Antibody SKU:
PACO22256
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from internal of human CBLN1.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from HUVEC cells, using CBLN1 antibody.
Background:
Required for synapse integrity and synaptic plasticity. During cerebellar synapse formation, essential for the formation and maintenance of parallel fiber and Purkinje cell synapses. When parallel fibers make contact with Purkinje spines, CBLN1 interaction with GRID2 triggers the recruitment of NRXN1 and secretory vesicles to the sites of contact. NRXN1-CBLN1-GRID2 signaling induces presynaptic morphological changes, which may further accumulate pre- and postsynaptic components to promote bidirectional maturation of parallel fiber - Purkinje cell functionnally active synapses by a positive feedback mechanism. Required for CBLN3 export from the endoplasmic reticulum and secretion By similarity.The cerebellin peptide exerts neuromodulatory functions. Directly stimulates norepinephrine release via the adenylate cyclase/PKA-dependent signaling pathway; and indirectly enhances adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release By similarity.
Synonyms:
Cerebellin-1; Precerebellin;
UniProt Protein Function:
CBLN1: Cerebellin-1 is required for synapse integrity and synaptic plasticity. Required for the ER export and secretion of CBLN3.Protein type: Secreted; Secreted, signal peptideChromosomal Location of Human Ortholog: 16q12.1Cellular Component: postsynaptic membrane; extracellular region; cell junctionMolecular Function: protein homodimerization activityBiological Process: heterophilic cell adhesion; positive regulation of synaptogenesis; synaptic transmission; nervous system development; protein secretion; cerebellar granule cell differentiation
UniProt Protein Details:
NCBI Summary:
This gene encodes a cerebellum-specific precursor protein, precerebellin, with similarity to the globular (non-collagen-like) domain of complement component C1qB. Precerebellin is processed to give rise to several derivatives, including the hexadecapeptide, cerebellin, which is highly enriched in postsynaptic structures of Purkinje cells. Cerebellin has also been found in human and rat adrenals, where it has been shown to enhance the secretory activity of this gland. [provided by RefSeq, Aug 2008]
