The CBL Polyclonal Antibody (CAB0732) is a valuable tool for researchers studying CBL, a crucial protein involved in cell signaling pathways and regulation. This antibody, generated in rabbits, demonstrates high reactivity with human samples and has been validated for use in Western blot applications. By binding to the CBL protein, this antibody enables precise detection and analysis in a variety of cell types, making it well-suited for studies in cancer research and cell signaling.CBL is known for its role in regulating cell growth, division, and differentiation, making it a key player in various cellular processes.
Dysregulation of CBL has been implicated in the development and progression of cancer, making it a promising target for therapeutic interventions. By studying the functions of CBL using this antibody, researchers can gain valuable insights into potential treatment strategies for cancer and other diseases linked to aberrant cell signaling pathways.
Product Name:
CBL Rabbit Polyclonal Antibody
SKU:
CAB0732
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 850-906 of human CBL (NP_005179.2).
WB,1:1000 - 1:5000 IHC-P,1:50 - 1:100 IF/ICC,1:100 - 1:500 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Synonyms:
CBL2; NSLL; C-CBL; RNF55; FRA11B; CBL
Positive Sample:
293T,Mouse thymus
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cytoplasm.
Calculated MW:
100kDa
Observed MW:
120kDa/110kDa
This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia, and expansion of CGG repeats in the 5' UTR has been associated with Jacobsen syndrome. Mutations in this gene are also the cause of Noonan syndrome-like disorder.
Purification Method:
Affinity purification
Gene ID:
867
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using CBL Rabbit pAb (CAB0732) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
