The CBFB Polyclonal Antibody (CAB5305) is a valuable tool for researchers studying the CBFB protein, a vital component in the regulation of gene expression and hematopoiesis. This antibody, produced in rabbits, shows high reactivity with human samples and is suitable for use in Western blot applications. By specifically binding to the CBFB protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it ideal for studies in molecular biology, oncology, and hematological research.
CBFB is essential for normal blood cell development and has been implicated in various hematological malignancies, making it an important target for therapeutic interventions. Understanding the role of CBFB in gene regulation and hematopoiesis is crucial for developing effective treatments for blood disorders and cancers. The CBFB Polyclonal Antibody provides researchers with a reliable tool for investigating the function and regulation of CBFB in health and disease.
Product Name:
CBFB Rabbit Polyclonal Antibody
SKU:
CAB5305
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-182 of human CBFB (NP_001746.1).
The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
865
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using CBFB Rabbit pAb (CAB5305) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
