The Cathepsin F Polyclonal Antibody (CAB10469) is a valuable tool for researchers studying Cathepsin F, a lysosomal cysteine protease involved in protein degradation and antigen processing. This highly reactive antibody is produced in rabbits and is specifically validated for use in Western blot applications with human samples.Cathepsin F plays an important role in immune regulation and has been implicated in various pathological conditions, making it a key target for research in immunology and cancer biology. This antibody binds specifically to the Cathepsin F protein, allowing for the detection and analysis of its expression in different cell types.
By understanding the role of Cathepsin F in immune responses and disease processes, researchers can uncover potential therapeutic targets for conditions such as cancer, autoimmune disorders, and inflammatory diseases. The use of the Cathepsin F Polyclonal Antibody provides a reliable tool for furthering our understanding of this important enzyme and its implications in various pathological conditions.
Product Name:
CTSF Rabbit Polyclonal Antibody
SKU:
CAB10469
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 270-484 of human CTSF (NP_003784.2).
Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region. The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains some of the elements known to be important for inhibitory activity. CTSF encodes a predicted protein of 484 amino acids which contains a 19 residue signal peptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The cathepsin F gene is ubiquitously expressed, and it maps to chromosome 11q13, close to the gene encoding cathepsin W.
Purification Method:
Affinity purification
Gene ID:
8722
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CTSF Rabbit pAb (CAB10469) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
