The Cathepsin B Monoclonal Antibody is a specialized antibody designed for research involving the Cathepsin B enzyme, a lysosomal cysteine protease that plays a crucial role in various biological processes. This antibody, developed and validated for use in Western blot and immunohistochemistry applications, specifically targets the Cathepsin B protein, enabling precise detection and analysis in a range of cell and tissue samples.Cathepsin B is known for its involvement in processes such as protein degradation, antigen processing, and apoptosis, making it a key player in cellular homeostasis and disease progression. The Cathepsin B Monoclonal Antibody offers researchers a powerful tool for studying the function and expression of Cathepsin B in diverse experimental settings, including studies related to cancer, inflammation, and neurodegenerative disorders.
By providing researchers with a reliable and highly specific tool for investigating Cathepsin B activity, this antibody contributes to advancing our understanding of the enzyme's role in health and disease. Its applications in both basic research and drug development make it an invaluable resource for scientists exploring the therapeutic potential of targeting Cathepsin B in various pathologies.
Product Name:
Cathepsin B Monoclonal Antibody
SKU:
CAB23039
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 8-339 of human Cathepsin B.
This gene encodes a member of the C1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to form the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer's disease, the most common cause of dementia. Overexpression of the encoded protein has been associated with esophageal adenocarcinoma and other tumors. Both Cathepsin B and Cathepsin L are involved in the cleavage of the spike protein from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) upon its entry to the human host cell. Multiple pseudogenes of this gene have been identified.
Purification Method:
Affinity purification
Gene ID:
1508
Clone Number:
ARC59069
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Hep G2 cells, using Cathepsin B Rabbit mAb (CAB23039) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Enhanced Kit (AbGn00021).Exposure time: 120s.
