The Catenin beta 1 Polyclonal Antibody (CAB11512) is a valuable tool for researchers studying Catenin beta 1, a crucial protein involved in cell adhesion and signaling pathways. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to Catenin beta 1, it enables researchers to detect and analyze the protein in a variety of cell types, making it a versatile tool for studies in cell biology and cancer research.
Catenin beta 1 is known to play a key role in cell adhesion and cell-cell communication, making it essential for processes such as development, tissue maintenance, and wound healing. Dysregulation of Catenin beta 1 has been implicated in various diseases, including cancer, where it can promote tumor growth and metastasis. By studying the function of Catenin beta 1, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets for intervention.
WB,1:100 - 1:500 IHC-P,1:20 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
EVR7; CTNNB; MRD19; NEDSDV; armadillo; β-Catenin
Positive Sample:
Mouse brain,Mouse lung,C6
Conjugate:
Unconjugated
Cellular Localization:
Cell junction, Cell membrane, Cytoplasm, Nucleus, adherens junction, centrosome, cytoskeleton, microtubule organizing center, spindle pole.
Calculated MW:
85kDa
Observed MW:
92kDa
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
1499
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using β-Catenin Rabbit pAb (CAB11512) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
