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Catalase Activity Assay Kit (MAES0044)

SKU:
MAES0044
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Cells, Cell Culture Supernatant, Tissue
Sensitivity:
1.12 U/mL
Range:
1.12 -150 U/mL
Assay Time:
40 min
Shipping:
Gel Pack
Research Area:
Oxidative Stress
Antioxidant
Diabetes & Obesity
Plant & Environmental Stress
Enzyme Activity
€259
Frequently bought together:

Description

system_update_altDatasheet
Product Name:Catalase Activity Assay Kit
Product Code:MAES0044
Product Size:96 Assays
Sample Type:Serum, plasma, cells, cell culture supernatant, tissue
Assay Time:40 min
Instrument:Microplate reader
Sensitivity:1.12 U/mL
Detection Range:1.12 -150 U/mL
Recovery Rate:100
Inter CV:7.7
Inter CV:3.9

CAT is an enzyme in organism that can efficiently and specifically decompose hydrogen peroxide and is a binding enzyme with iron porphyrin as an auxiliary group. CAT clears hydrogen peroxide in the body and protects cells from the toxicity of H2O2. CAT can also oxidize certain cytotoxic substances, such as formaldehyde, formic acid, phenol and ethanol. According to the difference of catalytic center structure, CAT can be divided into two types, one is iron porphyrin structure, also known as iron porphyrin enzyme, the other contain manganese ion, also known as manganese catalase. CAT is common in breathing organisms. It is mainly found in chloroplasts, mitochondria, endoplasmic reticulum, liver and red blood cells of animals.

The reaction that catalase (CAT) decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm.

Shelf Life:6 Months
Storage:2-8°C
Shipping:Gel Pack
Research Area:Enzymes, Oxidative stress

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