The CAST Polyclonal Antibody (CAB16789) is a valuable tool for researchers studying the CAST protein, a crucial component involved in immune regulation. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By binding to the CAST protein, researchers can effectively detect and analyze its presence in a variety of cell types, making it ideal for investigations in immunology and cancer research.
CAST, also known as calpastatin, plays an essential role in regulating immune responses by inhibiting immune reactions. Its function in immune system modulation makes it a promising target for research into diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions. With a deeper understanding of CAST activity, researchers can develop potential therapies that harness or modulate immune function to combat these diseases effectively.
Product Name:
CAST Rabbit Polyclonal Antibody
SKU:
CAB16789
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human CAST (NP_001177371.1).
The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
831
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using CAST Rabbit pAb (CAB16789) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
