Anti-CAST Antibody (CAB13683)
- SKU:
- CAB13683
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
Anti-CAST Antibody (CAB13683)
The CAST Polyclonal Antibody (CAB13683) is a valuable tool for researchers studying the calcium-sensitive protein named calpastatin (CAST). This polyclonal antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the CAST protein, this antibody allows for the detection and analysis of CAST in various cell types, making it an essential resource for studies in molecular biology and cell signaling pathways.Calpastatin, the protein targeted by this antibody, is known for its role in the regulation of calpain activity, a family of calcium-dependent proteases implicated in various cellular processes such as apoptosis, cell migration, and cytoskeletal remodeling.
Understanding the function of calpastatin is crucial for unraveling the complexities of these pathways and their dysregulation in diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases. The CAST Polyclonal Antibody provides researchers with a powerful tool to investigate the role of calpastatin in these conditions and potentially identify new therapeutic targets for intervention.
| Antibody Name: | Anti-CAST Antibody |
| Antibody SKU: | CAB13683 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 550-650 of human CAST (NP_001177371.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, NCI-H460, Mouse lung, Rat liver |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 550-650 of human CAST (NP_001177371.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SDKD LDDA LDKL SDSL GQRQ PDPD ENKP MEDK VKEK AKAE HRDK LGER DDTI PPEY RHLL DDNG QDKP VKPP TKKS EDSK KPAD DQDP IDAL SGDL DSCP S |
| Gene ID: | 831 |
| Uniprot: | P20810 |
| Cellular Location: | |
| Calculated MW: | 63kDa/71- 84kDa |
| Observed MW: | 130kDa |
| Synonyms: | CAST, BS-17, PLACK |
| Background: | The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. |
| UniProt Protein Function: | calpastatin: Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. Belongs to the protease inhibitor I27 (calpastatin) family. 7 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Inhibitor Chromosomal Location of Human Ortholog: 5q15 Cellular Component: membrane; cytoplasm; cytosol Molecular Function:protein binding; endopeptidase inhibitor activity; cysteine protease inhibitor activity Disease: Peeling Skin With Leukonychia, Acral Punctate Keratoses, Cheilitis, And Knuckle Pads |
| NCBI Summary: | The protein encoded by this gene is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The encoded protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010] |
| UniProt Code: | P20810 |
| NCBI GenInfo Identifier: | 126302556 |
| NCBI Gene ID: | 831 |
| NCBI Accession: | P20810.4 |
| UniProt Related Accession: | P20810 |
| Molecular Weight: | 63kDa; 71-84kDa |
| NCBI Full Name: | Calpastatin |
| NCBI Synonym Full Names: | calpastatin |
| NCBI Official Symbol: | CASTÂ Â |
| NCBI Official Synonym Symbols: | BS-17; PLACKÂ Â |
| NCBI Protein Information: | calpastatin |
| UniProt Protein Name: | Calpastatin |
| UniProt Synonym Protein Names: | Calpain inhibitor; Sperm BS-17 component |
| Protein Family: | Calpastatin |
| UniProt Gene Name: | CASTÂ Â |
| UniProt Entry Name: | ICAL_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CAST antibody (CAB13683) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using CAST antibody (CAB13683) at dilution of 1:100. Blue: DAPI for nuclear staining. |
Related Products
Anti-CAST Antibody (CAB16791)
Anti-CREB1 Antibody (CAB11063)
Anti-NUR77 Antibody (CAB0843)
Anti-HDAC6 Antibody (CAB11429)
Anti-GSK3Beta Antibody (CAB11360)
Anti-SQSTM1 Antibody (CAB11246)
