The Caspase-9 Polyclonal Antibody (CAB11451) is an essential tool for research involving caspase-9, a key player in the apoptotic pathway. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the caspase-9 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for studies in cell biology and cancer research.Caspase-9, also known as apoptotic protease Mch-6, is a critical component of the caspase family of proteins that regulate apoptosis, or programmed cell death. Dysregulation of caspase-9 activity has been implicated in various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions.
By studying the role of caspase-9 in these pathologies, researchers can uncover potential therapeutic targets and develop novel treatment strategies.The Caspase-9 Polyclonal Antibody is a valuable tool for investigating the function and regulation of caspase-9 in health and disease. Its high specificity and sensitivity make it a reliable choice for researchers looking to delve deeper into the molecular mechanisms underlying apoptosis and explore new avenues for therapeutic intervention.
Product Name:
Caspase-9 Rabbit Polyclonal Antibody
SKU:
CAB11451
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human Caspase-9 (NP_001220.2).
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
842
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using Caspase-9 Rabbit pAb (CAB11451) at 1:1000 dilution.L929 cells were treated by staurosporine(1 uM) for 3 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
