The Caspase-9 Polyclonal Antibody (CAB0281) is a valuable tool for researchers studying apoptosis, a process critical for normal development and the maintenance of tissue homeostasis. This antibody, produced by Assay Genie and raised in rabbits, is highly specific for human caspase-9 and is validated for use in Western blot applications. Caspase-9 is a key player in the intrinsic apoptotic pathway, acting as an initiator caspase that is activated in response to various cellular stress signals. Dysregulation of caspase-9 activity has been implicated in numerous diseases, including cancer, neurodegenerative disorders, and autoimmune conditions.
By utilizing the Caspase-9 Polyclonal Antibody in their research, scientists can gain valuable insights into the role of caspase-9 in health and disease. This antibody enables the detection and analysis of caspase-9 expression in a variety of cell types, making it an essential tool for studies in cell biology, molecular biology, and drug development. Understanding the mechanisms that govern caspase-9 activation and function is essential for developing targeted therapies that modulate apoptosis in disease states.
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
842
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Immunohistochemistry analysis of Caspase-9 in paraffin-embedded mouse kidney tissue using Caspase-9 Rabbit pAb (CAB0281) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
