The Caspase-8 Polyclonal Antibody (CAB11450) is a valuable tool for researchers studying apoptosis and cell death pathways. Caspase-8 is a key enzyme involved in the initiation of apoptosis, playing a crucial role in regulating programmed cell death. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.By targeting the Caspase-8 protein, this antibody enables researchers to study the expression and activation of this important enzyme in various cell types. Understanding the activity of Caspase-8 is essential for investigating its role in physiological processes such as development, as well as its implications in diseases like cancer and neurodegenerative disorders.
Overall, the Caspase-8 Polyclonal Antibody is a valuable tool for researchers interested in unraveling the complexities of cell death pathways and exploring potential therapeutic strategies targeting apoptosis. Its high reactivity and specificity make it an ideal choice for studies in apoptosis, cancer biology, and other related fields of research.
Product Name:
Caspase-8 Rabbit Polyclonal Antibody
SKU:
CAB11450
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human Caspase-8 (NP_203519.1).
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
Purification Method:
Affinity purification
Gene ID:
841
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Caspase-8 antibody (CAB11450) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
