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Caspase-8 Rabbit Polyclonal Antibody (CAB11450)

SKU:
CAB11450
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Caspase-8 Rabbit Polyclonal Antibody
SKU:CAB11450
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 300-400 of human Caspase-8 (NP_203519.1).
Sequence:QLMD HSNM DCFI CCIL SHGD KGII YGTD GQEA PIYE LTSQ FTGL KCPS LAGK PKVF FIQA CQGD NYQK GIPV ETDS EEQP YLEM DLSS PQTR YIPD EADF L
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8; Caspase-8
Positive Sample:Jurkat,Mouse liver,Rat thymus
Conjugate:Unconjugated
Cellular Localization:Cytoplasm.
Calculated MW:55kDa
Observed MW:57kDa

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Purification Method:Affinity purification
Gene ID:841
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using Caspase-8 antibody (CAB11450) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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