The Caspase-8 Polyclonal Antibody (CAB0215) is a valuable tool for researchers studying apoptosis and cell death mechanisms. Caspase-8 is a critical mediator of apoptosis, playing a key role in the initiation of the extrinsic apoptosis pathway. This antibody, produced in rabbits, has high reactivity with human samples and is validated for use in Western blot applications.By binding specifically to Caspase-8, this antibody allows for the detection and analysis of this important protein in a variety of cell types.
Its use is ideal for studies in cell biology, cancer research, and drug development, as Caspase-8 is implicated in various diseases and therapeutic strategies.Understanding the regulation and function of Caspase-8 is crucial for advancing knowledge of cell death pathways and developing targeted therapies for diseases such as cancer. The Caspase-8 Polyclonal Antibody is a powerful tool for researchers aiming to unravel the complexities of apoptosis and its role in disease progression.
Product Name:
Caspase-8 Rabbit Polyclonal Antibody
SKU:
CAB0215
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 376-479 of human Caspase 8 (NP_203519.1).
This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
Purification Method:
Affinity purification
Gene ID:
841
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using Caspase-8 Rabbit pAb (CAB0215) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
