The Caspase-3 Polyclonal Antibody (CAB16793) is specifically designed for research involving caspase-3, a key enzyme involved in apoptosis, or programmed cell death. This antibody, raised in rabbits, is highly reactive with human samples and has been validated for use in Western blot and immunohistochemistry applications. Caspase-3 is a crucial mediator of apoptosis in various cell types, making this antibody ideal for studies in cell biology, cancer research, and drug development. By targeting caspase-3, researchers can investigate the mechanisms underlying cell death and survival pathways, providing valuable insights into diseases such as cancer, neurodegenerative disorders, and autoimmune conditions.
Understanding the role of caspase-3 in apoptosis is essential for developing targeted therapies that promote cell death in cancer cells or prevent excessive cell death in neurodegenerative diseases. The Caspase-3 Polyclonal Antibody is a valuable tool for researchers aiming to unravel the intricate processes of cell death and survival in health and disease.
Product Name:
Caspase-3 Rabbit Polyclonal Antibody
SKU:
CAB16793
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 29-175 of human Caspase-3 (NP_004337.2).
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
CPP32; SCA-1; CPP32B; Caspase-3
Positive Sample:
HeLa,293T,NIH/3T3,Jurkat
Cellular Localization:
Cytoplasm.
Calculated MW:
32kDa
Observed MW:
17kDa/35kDa
The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Purification Method:
Affinity purification
Gene ID:
836
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Caspase-3 Rabbit pAb (CAB16793) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
