null

Caspase-3 Rabbit Polyclonal Antibody (CAB16793)

SKU:
CAB16793
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Caspase-3 Rabbit Polyclonal Antibody
SKU:CAB16793
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 29-175 of human Caspase-3 (NP_004337.2).
Sequence:SGIS LDNS YKMD YPEM GLCI IINN KNFH KSTG MTSR SGTD VDAA NLRE TFRN LKYE VRNK NDLT REEI VELM RDVS KEDH SKRS SFVC VLLS HGEE GIIF GTNG PVDL KKIT NFFR GDRC RSLT GKPK LFII QACR GTEL DCGI ETD
Tested Applications:WB IHC-P IF/ICC IP ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:CPP32; SCA-1; CPP32B; Caspase-3
Positive Sample:HeLa,293T,NIH/3T3,Jurkat
Cellular Localization:Cytoplasm.
Calculated MW:32kDa
Observed MW:17kDa/35kDa

The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.

Purification Method:Affinity purification
Gene ID:836
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using Caspase-3 Rabbit pAb (CAB16793) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.

Related Products