The Caspase-3 Polyclonal Antibody (CAB11040) is a valuable tool for researchers studying apoptosis and cell death processes. Caspase-3 is a key executioner caspase involved in the initiation and execution of apoptosis, making it a crucial target for therapeutic interventions in diseases such as cancer.This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. It binds specifically to the Caspase-3 protein, enabling precise detection and analysis in a variety of cell types.
Research involving Caspase-3 is essential for understanding the mechanisms of programmed cell death and for developing targeted therapies for diseases characterized by aberrant apoptosis pathways. The Caspase-3 Polyclonal Antibody is an indispensable tool for immunology and cancer research, providing valuable insights into the role of Caspase-3 in disease progression and treatment strategies.
The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.
Purification Method:
Affinity purification
Gene ID:
836
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of Jurkat cells, using Caspase-3 antibody (CAB11040) at 1:1000 dilution.Jurkat cells were treated by staurosporine(1 uM) for 3 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
