Caspase 3/7 Activity Assay Kit (AKES194)
- SKU:
- AKES194
- Product Type:
- Assay
- Detection Method:
- Colorimetric
- Instrument:
- Spectrophotometer
- Application:
- Caspase
Description
| Product Name: | Caspase 3/7 Activity Assay Kit |
| Product Code: | AKES194 |
| Sample Type: | Cell and tissue samples |
| Assay Time: | 4-6 hours |
| Assay Type: | Qualitative or Quantitative |
| Detection Instrument: | Spectrophotometer |
| Detection Method: | Colorimetric method |
| Application: | Caspase |
Caspase 3, also known as CPP32, Yama or apopain, belongs to the CED-3 subfamily of the caspase family. It is a key enzyme in the process of apoptosis and the most studied caspase in mammalian cells. Caspase 3 exists in the cytoplasm in the form of zymogen under normal condition and has no activity. In the stage of apoptosis, caspase 3 is activated, and the activated caspase 3 consists of two large subunits and two small subunits, which cleaves the corresponding cytoplasmic and nuclear substrates and ultimately leads to apoptosis. This caspase 3/7 activity assay kit is based on caspase 3/7 that can catalyze the substrate Ac-DEVD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 3/7 can be calculated by determining the absorbance at 405 nm.
| Storage: | -20°C, Shading light |
| Valid Period: | 12 months |
| Other Reagents Required: | PBS，Bradford Protein Determination Kit |
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