The Caspase-1 Polyclonal Antibody (CAB0964) is a valuable tool for researchers studying caspase-1, an important enzyme involved in inflammation and cell death. This antibody, produced in rabbits, is highly specific for detecting caspase-1 in human samples and is validated for use in Western blotting applications.Caspase-1 plays a key role in the inflammatory response by activating pro-inflammatory cytokines such as interleukin-1β. Dysregulation of caspase-1 activity has been implicated in various diseases, including autoimmune disorders and inflammatory conditions.
By using the Caspase-1 Polyclonal Antibody, researchers can accurately detect and analyze caspase-1 expression in different cell types, providing valuable insights into the role of this enzyme in health and disease. This antibody is an essential tool for studies in immunology, oncology, and inflammatory diseases, helping to further our understanding of caspase-1 and its potential as a therapeutic target.
Product Name:
Caspase-1 Rabbit Polyclonal Antibody
SKU:
CAB0964
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 201-300 of human Caspase 1 (NP_150634.1).
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms.
Purification Method:
Affinity purification
Gene ID:
834
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using CASP1 Rabbit pAb (CAB0964) at 1:1800 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
