The CASC5 Polyclonal Antibody (CAB13108) is a valuable tool for researchers studying cell division and chromosome segregation. CASC5, also known as Cancer susceptibility candidate 5, is a key protein involved in accurate chromosome segregation during cell division, making it essential for maintaining genomic stability.This polyclonal antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the CASC5 protein, this antibody enables the detection and analysis of CASC5 in various cell types, making it ideal for studies in cancer research and cell biology.
Understanding the role of CASC5 in cell division is crucial for unraveling the molecular mechanisms underlying chromosomal instability, a hallmark of many cancers. By exploring the functions of CASC5, researchers can gain valuable insights into potential therapeutic targets for cancer treatment and the development of novel strategies for combating tumor growth and progression.
Product Name:
CASC5 Rabbit Polyclonal Antibody
SKU:
CAB13108
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CASC5 (NP_733468.3).
The protein encoded by this gene is a component of the multiprotein assembly that is required for creation of kinetochore-microtubule attachments and chromosome segregation. The encoded protein functions as a scaffold for proteins that influence the spindle assembly checkpoint during the eukaryotic cell cycle and it interacts with at least five different kinetochore proteins and two checkpoint kinases. In adults, this gene is predominantly expressed in normal testes, various cancer cell lines and primary tumors from other tissues and is ubiquitously expressed in fetal tissues. This gene was originally identified as a fusion partner with the mixed-lineage leukemia (MLL) gene in t(11;15)(q23;q14). Mutations in this gene cause autosomal recessive primary microcephaly-4 (MCPH4). Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described but their biological validity has not been confirmed.
Purification Method:
Affinity purification
Gene ID:
57082
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using CASC5 Rabbit pAb (CAB13108) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
