The Cas9 Polyclonal Antibody (CAB14997) is a key tool for researchers working with the CRISPR/Cas9 system, a revolutionary technology used for genome editing. This antibody, produced in rabbits, is highly specific to Cas9 protein, enabling researchers to detect and analyze Cas9 expression in various cell types. Validated for use in Western blot applications, the Cas9 Polyclonal Antibody is essential for studies in molecular biology, genetics, and biotechnology.Cas9 is an RNA-guided endonuclease that plays a crucial role in the CRISPR/Cas9 system by creating double-strand breaks in DNA, allowing for precise modification of genes.
Understanding the activity and localization of Cas9 is essential for optimizing genome editing experiments and developing novel gene therapies. By using the Cas9 Polyclonal Antibody, researchers can accurately track Cas9 expression, ensuring successful genome editing outcomes in their studies.
Product Name:
CAS9 Rabbit Polyclonal Antibody
SKU:
CAB14997
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Other (Wide Range Predicted)
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 450-730 of streptococcus pyogenes m1 gas CAS9 (NP_269215.1).
Staphylococcus aureus Cas9 (SaCas9) is an RNA-guided endonuclease that targets complementary DNA adjacent to a protospacer adjacent motif (PAM) for cleavage. Its small size facilitates in vivo delivery for genome editing in various organisms.
Purification Method:
Affinity purification
Gene ID:
901176
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of 293T cells and 293T transfected with CAS9 protein, using CAS9 Rabbit pAb (CAB14997) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 120s.
