The Cas9 Monoclonal Antibody is a powerful tool for researchers studying CRISPR technology and genetic engineering. This antibody specifically targets the Cas9 protein, a key component of the CRISPR-Cas9 system responsible for precise gene editing. Produced through a unique monoclonal antibody production process, this antibody offers high specificity and sensitivity in detecting Cas9 in various experimental settings. It is suitable for use in Western blotting, immunofluorescence, and immunoprecipitation assays, providing researchers with reliable results for their studies.
Cas9 is widely used in gene editing applications for genetic engineering, disease modeling, and functional genomics research. By targeting Cas9 with this monoclonal antibody, researchers can accurately monitor its expression and localization in cells, aiding in the optimization and validation of CRISPR-mediated gene editing experiments.This Cas9 Monoclonal Antibody is a valuable tool for scientists exploring the potential of CRISPR technology in advancing our understanding of genetic mechanisms and developing innovative therapies for a wide range of diseases.
Product Name:
Cas9 Monoclonal Antibody
SKU:
CAB23005
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Other (Wide Range Predicted)
Immunogen:
Recombinant protein of streptococcus pyogenes m1 Cas9.
CRISPR-associated protein 9 (Cas9) is an RNA-guided DNA nuclease and is a component of the Streptococcus pyogenes CRISPR-antiviral immune system that provides adaptive immunity against extrachromosomal genetic material . The mechanism of CRISPR antiviral action involves three steps: (i) acquisition of foreign DNA by host bacteria; (ii) synthesis and maturation of CRISPR RNA (crRNA), followed by formation of RNA-Cas nuclease-protein complex; and (iii) acquisition of foreign DNA by the host bacterium; The complex recognizes foreign DNA and undergoes directed interference by cleavage by Cas nuclease activity . The type II CRISPR/Cas antiviral immune system provides a powerful tool for precise genome editing and has the potential for specific regulation of genes and therapeutic applications . The Cas9 protein and guide RNA (comprising a fusion between crRNA and transactivating crRNA (tracrRNA)) must be introduced or expressed in the cell. A 20-nucleotide sequence at the 5' end of the guide RNA guides Cas9 to a specific DNA target site. Thus, Cas9 can be "programmed" to cut a variety of DNA sites in vitro and in cells and organisms. The CRISPR/Cas9 genome editing tool has been used in many organisms, including mouse and human cells. Studies have shown that CRISPR can be used to generate mutant alleles or reporter genes in rodent and primate embryonic stem cells.
Purification Method:
Affinity purification
Gene ID:
901176
Clone Number:
ARC57839
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using Cas9 Rabbit mAb (CAB23005) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
