The CART Antibody (CAB5395) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and demonstrates high reactivity with human samples, making it suitable for use in various research applications such as Western blotting.CartPT is known for its essential role in maintaining the integrity and function of cartilage tissue. By targeting CartPT with this antibody, researchers can detect and analyze its expression in different cell types, providing valuable insights into its contributions to cartilage health and potential implications in diseases such as osteoarthritis.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
CART Antibody
SKU:
CAB5395
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CART
Positive Sample:
Mouse heart
Cellular Localization:
Secreted.
Calculated MW:
13kDa
Observed MW:
13kDa
This gene encodes a preproprotein that is proteolytically processed to generate multiple biologically active peptides. These peptides play a role in appetite, energy balance, maintenance of body weight, reward and addiction, and the stress response. Expression of a similar gene transcript in rodents is upregulated following administration of cocaine and amphetamine. Mutations in this gene are associated with susceptibility to obesity in humans.
Purification Method
Affinity purification
Gene ID
9607
RRID
AB_2766204
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from mouse heart, using CART Rabbit pAb (CAB5395) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
Immunofluorescence analysis of SH-SY5Y cells using CART Rabbit pAb (CAB5395) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
