The CARS Polyclonal Antibody (CAB6540) is a crucial tool for research involving the CARS protein, a key player in cancer metabolism. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the CARS protein, researchers can accurately detect and analyze its expression in various cell types, making it invaluable for studies in cancer biology and metabolism.
CARS, also known as cysteinyl-tRNA synthetase, plays a crucial role in tumor growth and metabolic reprogramming in cancer cells. By studying the function of CARS, researchers can gain valuable insights into the metabolic alterations that drive tumor formation and progression. This antibody is essential for understanding the role of CARS in cancer metabolism and developing targeted therapies that exploit these metabolic vulnerabilities.
Product Name:
CARS Rabbit Polyclonal Antibody
SKU:
CAB6540
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 469-748 of human CARS (NP_001742.1).
This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
833
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CARS antibody (CAB6540) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
