The CARIL2 Monoclonal Antibody (PACO62483) is a key tool for researchers studying CARIL2, a protein involved in immune cell activation and regulation. This antibody, derived from hybridoma cells, shows high specificity and sensitivity in detecting CARIL2 in human samples, making it an excellent choice for experiments such as Western blotting.CARIL2, also known as immune stimulatory molecule, plays a vital role in immune responses by promoting activation and proliferation of immune cells. Its involvement in enhancing immune function makes it an important target for research in immunology and cancer biology.
By studying CARIL2, researchers can gain insights into how immune cells are activated and regulated, potentially leading to the development of novel immunotherapies for conditions such as cancer and infectious diseases.In conclusion, the CARIL2 Monoclonal Antibody (PACO62483) is a valuable tool for researchers interested in understanding the role of CARIL2 in immune cell activation and regulation. Its high reactivity and specificity make it a reliable choice for studies in immunology and cancer research.
Western Blot. Positive WB detected in: Jurkat whole cell lysate. All lanes: CARMIL2 antibody at 1:1000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 155, 149 kDa. Observed band size: 155 kDa.
Immunoprecipitating CARMIL2 in Jurkat whole cell lysate. Lane 1: Rabbit control IgG instead of PACO62483 in Jurkat whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PACO62483 (6µg) + Jurkat whole cell lysate (500µg). Lane 3: Jurkat whole cell lysate (20µg).
Background:
Cell membrane-cytoskeleton-associated protein that plays a role in the regulation of actin polymerization at the barbed end of actin filaments. Prevents F-actin heterodimeric capping protein (CP) activity at the leading edges of migrating cells, and hence generates uncapped barbed ends and enhances actin polymerization. Plays a role in cell protrusion formations; involved in cell polarity, lamellipodial assembly, membrane ruffling and macropinosome formations. Involved as well in cell migration and invadopodia formation during wound healing.
Synonyms:
Capping protein, Arp2/3 and myosin-I linker protein 2 (Capping protein regulator and myosin 1 linker 2) (F-actin-uncapping protein RLTPR) (Leucine-rich repeat-containing protein 16C) (RGD, leucine-rich repeat, tropomodulin and proline-rich-containing protein), CARMIL2, LRRC16C RLTPR
UniProt Protein Function:
RLTPR: Belongs to the CARMIL family. 1 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 16q22.1Cellular Component: F-actin capping protein complex; membrane; cytoplasm; immunological synapse; intercellular junctionBiological Process: establishment of protein localization; cell migration; positive regulation of regulatory T cell differentiation; thymus development; homeostasis of number of cells; positive regulation of T cell proliferation; maintenance of cell polarity; T cell receptor signaling pathway
