The Card11 Polyclonal Antibody (CAB7140) is a key tool for researchers studying the CARD11 protein, an essential regulator of NF-κB signaling pathway activation in immune responses. This antibody, produced in rabbits, has high specificity for human samples and has been validated for use in Western blot assays. By binding to the CARD11 protein, this antibody facilitates the detection and analysis of CARD11 in various cell types, making it an invaluable tool for studies in immunology and cancer research.CARD11 is a crucial component of the adaptive immune response, playing a pivotal role in T cell receptor signaling and lymphocyte activation.
Dysregulation of CARD11 has been implicated in various autoimmune disorders, immunodeficiencies, and malignancies, highlighting its importance as a therapeutic target. Researchers aiming to unravel the complexities of immune signaling pathways and develop novel interventions can benefit greatly from the precise detection capabilities of the Card11 Polyclonal Antibody.
Product Name:
CARD11 Rabbit Polyclonal Antibody
SKU:
CAB7140
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 985-1154 of human CARD11 (NP_115791.3).
The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10.
Purification Method:
Affinity purification
Gene ID:
84433
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CARD11 antibody (CAB7140) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
