The Carboxypeptidase E Polyclonal Antibody (CAB5458) is a valuable tool for researchers studying carboxypeptidase E, an enzyme involved in the processing and maturation of peptide hormones and neuropeptides. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.Carboxypeptidase E plays a critical role in the regulation of neuropeptide levels, impacting various physiological processes such as pain perception, stress response, and energy balance.
Dysregulation of this enzyme has been implicated in conditions such as obesity, diabetes, and neurological disorders.The Carboxypeptidase E Polyclonal Antibody enables the detection and analysis of carboxypeptidase E protein levels in different cell types, making it ideal for studies in neuroscience, endocrinology, and metabolic disorders. Understanding the role of carboxypeptidase E in these contexts may provide insights into potential therapeutic targets for the treatment of related diseases.
Product Name:
CPE Rabbit Polyclonal Antibody
SKU:
CAB5458
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 257-476 of human CPE (NP_001864.1).
This gene encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. This protein may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in this gene are implicated in type 2 diabetes.
Purification Method:
Affinity purification
Gene ID:
1363
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using CPE antibody (CAB5458) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
