The CAR Carnosine ELISA Kit is specially designed for the precise measurement of carnosine levels in biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for various research studies.Carnosine is a naturally occurring dipeptide that plays a vital role in various physiological processes, including antioxidant defense and metal chelation.
Studies have shown that carnosine may have potential therapeutic benefits in conditions such as diabetes, neurodegenerative disorders, and cardiovascular diseases, making it a promising biomarker for further investigation and drug development.With the CAR Carnosine ELISA Kit, researchers can gain valuable insights into the role of carnosine in health and disease, paving the way for innovative treatments and interventions in the future.
Product Name:
Car (Carnosine) ELISA Kit (UNES00044)
SKU:
UNES00044
Target:
Car (Carnosine) (UNES00044)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Car. During the reaction, Car in the sample or standard competes with a fixed amount of Car on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Car. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Car in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
90-106
89-103
Average (%)
95
97
95
1:4
Range (%)
89-100
90-106
95-108
Average (%)
95
97
101
1:8
Range (%)
85-97
85-98
94-108
Average (%)
92
92
99
1:16
Range (%)
84-94
86-99
98-116
Average (%)
89
93
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
85-97
90
Cell culture media (n=5)
90-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.8
86.5
319.3
41.0
78.5
299.0
Standard deviation
2.6
4.2
16.0
2.2
3.8
10.2
C V (%)
6.07
4.86
5.01
5.37
4.84
3.41
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Car concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Car in samples. No significant cross-reactivity or interference between Car and analogues was observed.
