The CAPN3 Polyclonal Antibody (CAB12316) is a valuable tool for researchers studying the calpain-3 protein, which is involved in muscle function and maintenance. This antibody, derived from rabbits, shows high reactivity with human samples and is optimized for Western blot applications. By binding specifically to the CAPN3 protein, researchers can easily detect and analyze its expression in various cell types, making it an essential component for studies in muscular dystrophy, muscle physiology, and related fields.CAPN3, a member of the calpain family of proteins, plays a crucial role in muscle integrity and function by regulating protein turnover and maintaining muscle structure.
Mutations in the CAPN3 gene have been linked to various forms of muscular dystrophy, making it a key target for understanding and potentially treating these debilitating conditions. This antibody provides researchers with a powerful tool to explore the role of CAPN3 in muscle health and disease, aiding in the development of therapeutic strategies for muscular dystrophy and related disorders.
Product Name:
CAPN3 Rabbit Polyclonal Antibody
SKU:
CAB12316
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human CAPN3 (NP_775111.1).
Calpain, a heterodimer consisting of a large and a small subunit, is a major intracellular protease, although its function has not been well established. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed.
Purification Method:
Affinity purification
Gene ID:
825
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using CAPN3 Rabbit pAb (CAB12316) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
