The CAPN12 Polyclonal Antibody (PAC02218) is a valuable tool for researchers studying CAPN12, a member of the calpain family of proteases that play a role in various cellular processes. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the CAPN12 protein, this antibody enables the detection and analysis of CAPN12 in different cell types, making it ideal for research in the fields of molecular biology and cell biology.
CAPN12 is known for its involvement in processes such as cell proliferation, differentiation, and apoptosis, making it a promising target for investigations into diseases like cancer and neurodegenerative disorders. Understanding the function of CAPN12 is essential for unraveling its role in these conditions and potentially developing therapies that target this protease. With its reliability and specificity, the CAPN12 Polyclonal Antibody is a valuable asset for researchers looking to delve into the intricacies of CAPN12 biology.
Antibody Name:
CAPN12 Antibody (PACO22218)
Antibody SKU:
PACO22218
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from internal of human CAPN12.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from NIH-3T3 cells, A549 cells and K562 cells, using CAPN12 antibody.
Background:
Calcium-regulated non-lysosomal thiol-protease By similarity.
CAPN12: Calcium-regulated non-lysosomal thiol-protease. Belongs to the peptidase C2 family.Protein type: EC 3.4.22.-; ProteaseChromosomal Location of Human Ortholog: 19q13.2Cellular Component: cytoplasmMolecular Function: calcium-dependent cysteine-type endopeptidase activity; calcium ion bindingBiological Process: proteolysis
UniProt Protein Details:
NCBI Summary:
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes a member of the calpain large subunit family. [provided by RefSeq, Jun 2012]
