The CAPN1 Polyclonal Antibody (CAB1172) is specifically designed for research involving CAPN1, a calpain protease that plays a crucial role in various cellular processes, including cell proliferation, differentiation, and apoptosis. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in Western blot applications. It binds specifically to the CAPN1 protein, allowing for accurate detection and analysis in a variety of cell types, making it an ideal tool for studies in molecular biology and cell signaling pathways.
CAPN1, also known as mu-calpain, is involved in the regulation of numerous cellular functions, making it a key player in diseases such as cancer, neurodegenerative disorders, and muscular dystrophy. Understanding the mechanisms and pathways in which CAPN1 operates is essential for developing targeted therapies and treatments for these conditions. The CAPN1 Polyclonal Antibody is a valuable tool for researchers looking to unravel the complexities of CAPN1 and its implications in various diseases.
Product Name:
Calpain 1 Rabbit Polyclonal Antibody
SKU:
CAB1172
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 355-530 of human Calpain 1 (NP_005177.2).
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
823
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Calpain 1 Rabbit pAb (CAB1172) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
