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Canine Vascular Endothelial Growth Factor A (VEGFA) ELISA Kit

SKU:
CNDL00094
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9MYV3
Sensitivity:
5.4pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
VEGF,VEGF-A,VPF,Vascular Endothelial Cell Growth Factor A,Vascular Permeability Factor
Reactivity:
Canine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

system_update_altDatasheet

Canine Vascular Endothelial Growth Factor A (VEGFA) ELISA Kit

The Canine Vascular Endothelial Growth Factor-A (VEGFA) ELISA Kit from Assay Genie is a cutting-edge tool designed for the accurate measurement of VEGFA levels in canine serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing precise and consistent results for a variety of research applications.VEGFA is a key factor involved in angiogenesis, playing a critical role in promoting the formation of blood vessels and influencing cell growth.

Its implications in various health conditions such as cancer, cardiovascular diseases, and neurological disorders make it a vital biomarker for investigating these diseases and exploring potential therapeutic interventions.Trust Assay Genie's Canine VEGFA ELISA Kit to provide you with the reliable data you need to advance your research and contribute to a better understanding of canine health and physiology.

Product Name:Canine Vascular Endothelial Growth Factor A (VEGFA) ELISA Kit
Product Code:CNDL00094
Size:96 Assays
Target:VEGFA
Synonyms:VEGF, VEGF-A, VPF, Vascular Endothelial Cell Growth Factor A, Vascular Permeability Factor
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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