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Canine Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit

SKU:
CNDL00082
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
F1PKH2
Sensitivity:
5.4pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
TGF-B2,TGF-Beta2,G-TSF,LAP,Cetermin,Polyergin,Latency-associated peptide,BSC-1 cell growth inhibitor,Glioblastoma-derived T-cell suppressor factor
Reactivity:
Canine
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Canine Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit

The Canine Transforming Growth Factor Beta 2 (TGFB2) ELISA Kit is specifically designed for the precise measurement of TGFB2 levels in canine serum, plasma, and cell culture supernatants. This kit is engineered with advanced technology to deliver superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.TGFB2 is a key cytokine involved in regulating cellular processes such as cell growth, differentiation, and immune response. Dysregulation of TGFB2 has been linked to various diseases, including cancer, fibrosis, and inflammatory disorders, highlighting its importance as a potential therapeutic target and diagnostic marker.

By utilizing the Canine TGFB2 ELISA Kit, researchers can gain valuable insights into the role of TGFB2 in canine health and disease, paving the way for advancements in canine medicine and treatment strategies. Trust in the precision and reliability of this ELISA kit to accelerate your research and drive innovation in canine biology and veterinary medicine.

Product Name:Canine Transforming Growth Factor Beta 2 (TGFb2) ELISA Kit
Product Code:CNDL00082
Size:96 Assays
Target:TGFb2
Synonyms:TGF-B2, TGF-Beta2, G-TSF, LAP, Cetermin, Polyergin, Latency-associated peptide, BSC-1 cell growth inhibitor, Glioblastoma-derived T-cell suppressor factor
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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