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Canine Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

SKU:
CNDL00081
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P54831
Sensitivity:
5.6pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
TGF-B1,CED,DPD1,LAP,Camurati-Engelmann Disease,Latency-associated peptide
Reactivity:
Canine
Sample Type:
Serum, Platelet-Poor Plasma, Tissue Homogenates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Canine Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit

The Canine Transforming Growth Factor Beta 1 (TGFβ1) ELISA Kit is a reliable and accurate tool for measuring levels of TGFβ1 in canine serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures precise and reproducible results, making it suitable for a variety of research applications.TGFβ1 is a key regulator in canine biology, playing a crucial role in immune response, cell growth, and development.

Dysregulation of TGFβ1 has been implicated in various diseases such as cancer, inflammatory disorders, and fibrosis, highlighting its importance as a biomarker for understanding disease processes and potential therapeutic interventions.By using the Canine TGFβ1 ELISA Kit, researchers can gain valuable insights into the role of TGFβ1 in canine health and disease, paving the way for advancements in veterinary medicine and animal health.

Product Name:Canine Transforming Growth Factor Beta 1 (TGFb1) ELISA Kit
Product Code:CNDL00081
Size:96 Assays
Target:TGFb1
Synonyms:TGF-B1, CED, DPD1, LAP, Camurati-Engelmann Disease, Latency-associated peptide
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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