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Canine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit

SKU:
CNDL00084
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9TTY1
Sensitivity:
1.5ng/mL
Range:
6.25-400ng/mL
ELISA Type:
Sandwich
Synonyms:
CSC-21K,TIMP Metallopeptidase Inhibitor 2
Reactivity:
Canine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

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Canine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit

The Canine Tissue Inhibitors of Metalloproteinase-2 (TIMP2) ELISA Kit is a powerful tool for the precise measurement of TIMP2 levels in canine tissue samples, serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.TIMP2 is a key regulator of matrix metalloproteinases (MMPs), which play a crucial role in tissue remodeling and extracellular matrix degradation. Aberrant TIMP2 levels have been implicated in various diseases, including cancer, cardiovascular disorders, and inflammatory conditions.

By detecting and quantifying TIMP2, researchers can gain valuable insights into disease mechanisms and potentially identify novel therapeutic targets.Whether studying canine diseases or investigating the role of TIMP2 in tissue homeostasis, the Canine TIMP2 ELISA Kit offers unparalleled performance and reliability, making it an indispensable tool for researchers in the field of veterinary and biomedical sciences.

Product Name:Canine Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) ELISA Kit
Product Code:CNDL00084
Size:96 Assays
Target:TIMP2
Synonyms:CSC-21K, TIMP Metallopeptidase Inhibitor 2
Detection Method:Sandwich
Range:6.25-400ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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