Canine T Cell Response Array (13 targets) (SARB0011)
- SKU:
- SARB0011
- Product Type:
- Protein Array
- Analytes:
- GM-CSF
- IFN-gamma
- IL-1 alpha (IL-1 F1)
- IL-1 beta (IL-1 F2)
- IL-1 Ra (IL-1 F3)
- IL-10
- IL-12 p40
- IL-13
- IL-17A
- IL-21
- IL-6
- NCAM-1 (CD56)
- TNF alpha
- Reactivity:
- Canine
- Applications:
- Multiplex Array
Description
Canine T Cell Response Array (13 targets) (SARB0011)
The Canine T Cell Response Array (SARB0011) is a powerful tool for studying canine immune responses in research settings. This array contains 13 targets, allowing for comprehensive analysis of T cell responses in dogs. The array is designed to provide detailed insights into the activation and function of T cells in canine samples.This canine T cell response array enables researchers to simultaneously measure the expression levels of multiple key proteins involved in T cell activation and regulation. By profiling the T cell response in dogs, researchers can gain a better understanding of the immune system in canines and how it responds to various stimuli.
With its high sensitivity and specificity, this canine T cell response array is ideal for studies in immunology, veterinary medicine, and infectious diseases. Whether investigating immune responses in healthy dogs or studying the immune dysfunction in canine diseases, this array provides valuable information for a wide range of research applications.Overall, the Canine T Cell Response Array (SARB0011) is a valuable tool for researchers interested in studying T cell responses in dogs. Its comprehensive design and reliable performance make it an essential resource for advancing our understanding of canine immunology.
| Product Sku: | SARB0011 |
| Size: | 2, 4, or 8 |
| Species Detected: | Canine |
| Number of Targets Detected: | 13 |
| Gene Symbols: | CSF2, IFNG, IL10, IL12B, IL17A, IL1B, IL6, TNF |
| Compatible Sample Types: | Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates |
| Design Principle: | Sandwich-based |
| Method of Detection: | Chemiluminescence |
| Pathway: | |
| Quantitative/Semi-Quantitative: | Semi-Quantitative |
| Solid Support: | Membrane |
| Suggested Application(s): | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Storage/Stability: | For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months. |
| Hover over target for more information | ||||
|---|---|---|---|---|
GM-CSF
| IFN-gamma
| IL-1 alpha (IL-1 F1) | IL-1 beta (IL-1 F2)
| IL-1 Ra (IL-1 F3) |
IL-10
| IL-12 p40
| IL-13 | IL-17A
| IL-21 |
IL-6
| NCAM-1 (CD56)
| TNF alpha
| ||
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
- Canine T Cell Response Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
| 1. | Block membranes |
| 2. | Incubate with Sample |
| 3. | Incubate with Biotinylated Detection Antibody Cocktail |
| 4. | Incubate with HRP-Conjugated Streptavidin |
| 5. | Incubate with Detection Buffers |
| 6. | Image with chemiluminescent imaging system |
| 7. | Perform densitometry and analysis |
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