Canine PTH (Parathyroid hormone) ELISA Kit (AEFI00039)
- SKU:
- AEFI00039
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Sensitivity:
- 1.875pg/ml
- Range:
- 3.12-200pg/ml
- ELISA Type:
- Sandwich
- Reactivity:
- Canine
Description
Canine PTH (Parathyroid hormone) ELISA Kit
The Canine PTH (Parathyroid Hormone) ELISA Kit from Assay Genie is a cutting-edge solution for accurate detection of parathyroid hormone levels in canine serum, plasma, and other biological samples. With its exceptional sensitivity and specificity, this ELISA kit delivers precise and reliable results, making it a valuable tool for various research applications in veterinary medicine and canine health studies.Parathyroid hormone (PTH) is a vital regulatory hormone that plays a key role in calcium and phosphorus homeostasis, influencing bone metabolism and mineral balance. Abnormal PTH levels are associated with several health conditions in canines, including hyperparathyroidism, renal disease, and metabolic bone disorders, making it a critical biomarker for diagnosing and monitoring these diseases.
By using the Canine PTH ELISA Kit, researchers and veterinarians can access critical data to better understand canine endocrine health, bone metabolism, and mineral disorders, ultimately leading to improved diagnostics, treatment strategies, and overall canine well-being. Trust in Assay Genie's expertise and innovation to drive your canine research forward.
| Product Name: | Canine PTH(Parathyroid hormone) ELISA Kit |
| Product Code: | AEFI00039 |
| Size: | 96T |
| Alias: | Parathyroid hormone, PTH, Parathyrin |
| Detection method: | Sandwich ELISA, Double Antibody |
| Application: | PTH ELISA Kit allows for the in vitro quantitative determination of PTH concentrations in serum, plasma, tissue homogenates and other biological fluids. |
| Sensitivity: | < 1.875pg/ml |
| Range: | 3.12-200pg/ml |
| Storage: | 2-8°C for 6 months |
| Note: | For Research Use Only |
| Recovery: | Matrices listed below were spiked with certain level of PTH and the recovery rates were calculated by comparing the measured value to the expected amount of PTH in samples. | ||||||||||||||||
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| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PTH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. | ||||||||||||||||
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| CV(%): | Intra-Assay: CV<8% Inter-Assay: CV<10% |
When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.
| Sample Type | Protocol |
| Serum | If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. |
| Plasma | Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit. |
| Urine & Cerebrospinal Fluid | Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid. |
| Cell culture supernatant | Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately. |
| Cell lysates | Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Tissue homogenates | The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C. |
| Tissue lysates | Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Breast Milk | Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles. |
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