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Canine Monocyte Chemotactic Protein 4 (MCP4) ELISA Kit

SKU:
CNDL00064
Product Type:
ELISA Kit
Sensitivity:
7.1pg/mL
Range:
15.6-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CCL13,SCYA13,NCC1,SCYL1,CKb10,Chemokine C-C-Motif Ligand 13,Small Inducible Cytokine Subfamily A(Cys-Cys)Member 13,CK-beta-10,Monocyte chemoattractant 4
Reactivity:
Canine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Canine Monocyte Chemotactic Protein 4 (MCP4) ELISA Kit

The Canine Monocyte Chemotactic Protein 4 (MCP4) ELISA Kit is a valuable tool for researchers studying the role of MCP4 in canine health and disease. This kit is specifically designed for the accurate detection of MCP4 levels in canine serum, plasma, and cell culture supernatants with high sensitivity and specificity.MCP4 is a key chemokine involved in immune response and inflammation, attracting monocytes to sites of infection or tissue damage. Understanding the levels of MCP4 in canine samples can provide insight into the immune system's response to various conditions, such as infections, inflammatory diseases, and cancer.

With reliable and reproducible results, the Canine MCP4 ELISA Kit is suitable for a wide range of research applications, enabling researchers to further explore the role of MCP4 in canine health and potentially develop new diagnostic tools or therapies for relevant diseases.

Product Name:Canine Monocyte Chemotactic Protein 4 (MCP4) ELISA Kit
Product Code:CNDL00064
Size:96 Assays
Target:MCP4
Synonyms:CCL13, SCYA13, NCC1, SCYL1, CKb10, Chemokine C-C-Motif Ligand 13, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 13, CK-beta-10, Monocyte chemoattractant 4
Detection Method:Sandwich
Range:15.6-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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