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Canine Matrix Metalloproteinase 9 (MMP9) ELISA Kit

SKU:
CNDL00069
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q71U09
Sensitivity:
1.25ng/mL
Range:
3.125-200ng/mL
ELISA Type:
Sandwich
Synonyms:
GELB,Gelatinase B,CLG4B,CLG4-B,92 KDa Gelatinase,92kDa Type IV Collagenase
Reactivity:
Canine
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Canine Matrix Metalloproteinase 9 (MMP9) ELISA Kit

The Canine Matrix Metalloproteinase-9 (MMP9) ELISA Kit is specifically designed for the precise detection of MMP9 levels in canine serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.MMP9 is an important enzyme involved in extracellular matrix remodeling and tissue repair processes. Dysregulation of MMP9 activity has been implicated in various pathological conditions such as cancer, inflammatory diseases, and tissue damage, making it a valuable biomarker for understanding these conditions and exploring potential treatment strategies.

Overall, the Canine MMP9 ELISA Kit provides researchers with a reliable tool for studying MMP9 biology in canine samples, offering insights into the role of this enzyme in disease processes and potential therapeutic interventions.

Product Name:Canine Matrix Metalloproteinase 9 (MMP9) ELISA Kit
Product Code:CNDL00069
Size:96 Assays
Target:MMP9
Synonyms:GELB, Gelatinase B, CLG4B, CLG4-B, 92 KDa Gelatinase, 92kDa Type IV Collagenase
Detection Method:Sandwich
Range:3.125-200ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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