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Canine Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit

SKU:
CNDL00066
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q68AP1
Sensitivity:
6.1pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CCL20,CKb4,LARC,MIP3-A,SCYA20,ST38,Small Inducible Cytokine Subfamily A(Cys-Cys)Member 20,Beta-Chemokine Exodus-1,Liver And Activation-Regulated Chemokine
Reactivity:
Canine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Canine Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit

The Canine Macrophage Inflammatory Protein 3 Alpha (MIP-3α) ELISA Kit is specifically designed for the accurate measurement of MIP-3α levels in canine serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.MIP-3α is a key chemokine involved in the regulation of inflammatory responses and immune cell recruitment.

It plays a crucial role in the immune system's defense against infections and inflammatory diseases. The Canine MIP-3α ELISA Kit is an essential tool for studying the role of MIP-3α in canine health and disease, enabling researchers to better understand the mechanisms underlying immune responses and develop potential therapeutic interventions.

Product Name:Canine Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit
Product Code:CNDL00066
Size:96 Assays
Target:MIP3a
Synonyms:CCL20, CKb4, LARC, MIP3-A, SCYA20, ST38, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 20, Beta-Chemokine Exodus-1, Liver And Activation-Regulated Chemokine
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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