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Canine Luteinizing Hormone (LH) ELISA Kit

SKU:
CNDL00059
Product Type:
ELISA Kit
Sensitivity:
0.189ng/mL
Range:
0.468-30ng/mL
ELISA Type:
Competitive
Synonyms:
ICSH,Lutropin,Interstitial Cell Stimulating Hormone
Reactivity:
Canine
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Canine Luteinizing Hormone (LH) ELISA Kit

The Canine Luteinizing Hormone (LH) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of LH levels in canine serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for research in the field of reproductive endocrinology in dogs.LH is a key hormone involved in the regulation of the estrous cycle in female dogs and plays a crucial role in the process of ovulation.

Monitoring LH levels is essential for understanding reproductive function and can be used for diagnosing infertility, monitoring breeding programs, and evaluating reproductive health in canines.With its high sensitivity and specificity, the Canine LH ELISA Kit provides researchers with a valuable tool for studying canine reproductive physiology and developing strategies for improving reproductive outcomes in dogs.

Product Name:Canine Luteinizing Hormone (LH) ELISA Kit
Product Code:CNDL00059
Size:96 Assays
Target:LH
Synonyms:ICSH, Lutropin, Interstitial Cell Stimulating Hormone
Detection Method:Competition
Range:0.468-30ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)82-10292
EDTA plasma (n=5)90-10095
Heparin plasma (n=5)88-10094
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.Aspirate and wash 3 times
4.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.Aspirate and wash 5 times
6.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.Add 50µL Stop Solution. Read at 450 nm immediately.

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