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Canine Interleukin 6 (IL-6) ELISA Kit

SKU:
CNDL00054
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P41323
Sensitivity:
5.5pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
MGI2-A,MGI2A,HGF,BSF2,HSF,IFNB2,B-Cell Stimulatory Factor-2,Hybridoma/Plasmacytoma Growth Factor,Hepatocyte Stimulating Factor,Cytotoxic T-Cell Differentiation Factor
Reactivity:
Canine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Canine Interleukin 6 (IL-6) ELISA Kit

The Canine Interleukin-6 (IL-6) ELISA Kit from Assay Genie is specifically designed for the accurate detection of IL-6 levels in canine serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results, making it perfect for a wide range of research applications.IL-6 is a key cytokine involved in inflammatory responses and immune regulation. Elevated levels of IL-6 have been associated with various diseases and conditions in dogs, making it an important biomarker for studying these conditions and developing potential therapies.

Overall, the Canine IL-6 ELISA Kit provides researchers with a valuable tool for studying IL-6 levels in canine samples, advancing our understanding of inflammatory processes and immune responses in dogs. Visit the Assay Genie website for more information and to order your kit today.

Product Name:Canine Interleukin 6 (IL-6) ELISA Kit
Product Code:CNDL00054
Size:96 Assays
Target:IL6
Synonyms:MGI2-A, MGI2A, HGF, BSF2, HSF, IFNB2, B-Cell Stimulatory Factor-2, Hybridoma/Plasmacytoma Growth Factor, Hepatocyte Stimulating Factor, Cytotoxic T-Cell Differentiation Factor
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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