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Canine Intercellular Adhesion Molecule 1 (ICAM1) ELISA Kit

SKU:
CNDL00037
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P33729
Sensitivity:
0.064ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD54,BB2,P3.58,Human Rhinovirus Receptor,Major group rhinovirus receptor
Reactivity:
Canine
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

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Canine Intercellular Adhesion Molecule 1 (ICAM1) ELISA Kit

The Canine Intercellular Adhesion Molecule-1 (ICAM1) ELISA Kit is a powerful tool for the precise measurement of ICAM1 levels in canine serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research applications.ICAM1 is a key protein that plays a crucial role in immune response and inflammation, making it a valuable biomarker for studying conditions such as autoimmune diseases, infections, and inflammatory disorders.

By accurately measuring ICAM1 levels, researchers can gain valuable insights into these conditions and potentially develop novel therapeutic approaches.Overall, the Canine ICAM1 ELISA Kit is an indispensable tool for researchers interested in exploring the role of ICAM1 in canine health and disease, offering reliable and reproducible results to further advance scientific knowledge in this area.

Product Name:Canine Intercellular Adhesion Molecule 1 (ICAM1) ELISA Kit
Product Code:CNDL00037
Size:96 Assays
Target:ICAM1
Synonyms:CD54, BB2, P3.58, Human Rhinovirus Receptor, Major group rhinovirus receptor
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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