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Canine Coagulation Factor VIII (F8) ELISA Kit

SKU:
CNDL00028
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O18806
Sensitivity:
1.15ng/mL
Range:
3.125-200ng/mL
ELISA Type:
Sandwich
Synonyms:
FVIII,AHF,HEMA,F8B,F8C,Antihemophilic Globulin,Hemophilia A,Anti-Hemophilic Factor A,FVIII Procoagulant Component,F8 Protein
Reactivity:
Canine
Sample Type:
Plasma And Other Biological Fluids
€749
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Description

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Canine Coagulation Factor VIII (F8) ELISA Kit

The Canine Coagulation Factor VIII (F8) ELISA Kit is specifically designed for the accurate detection of coagulation factor VIII levels in canine serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Coagulation factor VIII is a key protein involved in the blood clotting process, playing a critical role in maintaining hemostasis. Dysregulation of factor VIII levels can lead to bleeding disorders such as hemophilia A, making it an important biomarker for studying coagulation disorders in dogs and potential treatment options.

Whether you are studying canine coagulation pathways, investigating bleeding disorders in dogs, or developing new therapies, the Canine Coagulation Factor VIII (F8) ELISA Kit provides a reliable tool for accurate quantitative analysis of factor VIII levels in canine samples.

Product Name:Canine Coagulation Factor VIII (F8) ELISA Kit
Product Code:CNDL00028
Size:96 Assays
Target:F8
Synonyms:F8, FVIII, AHF, HEMA, F8B, F8C, Antihemophilic Globulin, Hemophilia A, Anti-Hemophilic Factor A, FVIII Procoagulant Component, F8 Protein
Detection Method:Sandwich
Range:3.125-200ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)82-10292
EDTA plasma (n=5)90-10095
Heparin plasma (n=5)88-10094
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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